Derivatization of Amino Acids, Peptides and Proteins for Laser-Induced Fluorescence Detection in Capillary Electrophoresis

Authors

  • M. Ryvolová Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno
  • P. Táborský Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno
  • P. Vrábel Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno
  • J. Havel Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno
  • J. Preisler Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno

Abstract

This work is focused on application of laser-induced fluorescence detection for analysis of amino acids, peptides and proteins. The measurements were performed on a home-built instrument with a frequency-doubled Nd:YAG laser (532 nm) as excitation source. Rhodamine B isothiocyanate was used for derivatization of model samples. Major differences between the detection limits of analytes derivatized at high and low concentrations were presented. Difficulties associated with derivatization of complex samples and their negative influence on the limits of detection were also demonstrated.

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Published

2006-04-15

How to Cite

Ryvolová, M., Táborský, P., Vrábel, P., Havel, J., & Preisler, J. (2006). Derivatization of Amino Acids, Peptides and Proteins for Laser-Induced Fluorescence Detection in Capillary Electrophoresis. Chemické Listy, 100(3). Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1943

Issue

Vol. 100 No. 3 (2006)

Section

Articles